| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Cupriavidus necator | AGTGGTTCAACGACGCCA | GGCCTGAATCTTGGTGGCT | 59.50 | 60.00 | 179 | 128 |
100.00%
|
100.00%
|
| Primer 2 | Cupriavidus necator | GACCTCCGACATCGTGCAT | TGCCGAACTGCCGATCAT | 59.86 | 59.42 | 190 | 88 |
100.00%
|
100.00%
|
| Primer 3 | Cupriavidus necator | ACCTCCGACATCGTGCATG | TGCCGAACTGCCGATCAT | 60.15 | 59.42 | 189 | 84 |
100.00%
|
100.00%
|
| Primer 4 | Cupriavidus necator | ATGACAGCCAGCACGCAA | GATTCCCTGCACGCCAAAC | 60.28 | 59.79 | 216 | 74 |
100.00%
|
100.00%
|
| Primer 5 | Cupriavidus necator | ATGACAGCCAGCACGCAA | ATTCCCTGCACGCCAAAC | 60.28 | 58.63 | 215 | 74 |
100.00%
|
100.00%
|
| Primer 6 | Cupriavidus necator | GCATGGTCGAGATCTGGCA | TGGCGACATGCTTGACCA | 59.86 | 59.57 | 237 | 74 |
100.00%
|
100.00%
|
| Primer 7 | Cupriavidus necator | ACGGCATGGTCGAGATCTG | TGGCGACATGCTTGACCA | 59.56 | 59.57 | 240 | 74 |
100.00%
|
100.00%
|
| Primer 8 | Cupriavidus necator | ATGGCAGGCATCAGCACA | ACAGCAACGGCACATGGT | 59.64 | 60.20 | 298 | 72 |
100.00%
|
100.00%
|
| Primer 9 | Cupriavidus necator | TGAACGTGACCGCTTCCA | TGACGTTGGGCATCACCA | 59.18 | 59.17 | 231 | 68 |
100.00%
|
100.00%
|
| Primer 10 | Cupriavidus necator | TGCAGATGGAACTGTCGGC | AGCGCAAACAGCACGTTG | 60.38 | 59.98 | 166 | 65 |
100.00%
|
100.00%
|